Ana-Zyme G – Biocatalyst for anaerobic breakdown of FOG

Ana-Zyme G is formulated to provide immediate reduction of fat, oil, and grease in anaerobic digesters and lagoons to avoid issues with grease caps and foaming. The biocatalysts in Ana-Zyme G rapidly speed the breakdown of complex fat molecules into simple short-chain fatty acids which then break down to acetic acid, a compound used in methanogenesis.

The goal of this product is to limit volatile acid accumulation, while also increasing biogas generation, and improving volatile solids destruction.

Ana-Zyme G can degrade a range of fatty acids such as linolenic, oleic, stearic, and palmitic as well as fish oils, triglycerides, dairy, and egg fat. The production of short-chain fatty acids is very important as part of the acidogenesis–methanogenesis process.

Aquafix laboratories partners with those who run or operate anaerobic digesters of any kind through technical consulting and biological methane potential testing. Feel free to reach out to us for technical assistance any time.

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Ana-Zyme G Applications

    Anaerobic digesters/lagoons for:

  • Dairy processing
  • Meat processing
  • High fat food processing

Ana-Zyme G Benefits

  • Eliminates grease caps
  • Avoid foaming due to fats, oils and greases
  • Prevents buildup of fatty acids
  • Improves VA:ALK Ratio

The diagram to the left represents how Ana-Zyme G completes the β-Oxidation of Lauric Acid (a saturated fatty acid) into Capric Acid and Acetic Acid. All fatty acids are eventually converted 2 carbons at a time into acetic acid, which is the ideal food for methanogens. Therefore, fats—if degraded properly—can be an excellent food substrate in anaerobic systems.

High Levels of Grease In Anaerobic Digesters

FOG must undergo several steps to be converted into acetate, the ideal food substrate for methanogens: disintegration, hydrolysis, and β-oxidation.

Limitations in the rates of any of these steps can lead to uneven degradation of FOG, which results in variable volatile acid loading on methanogens. Variable loading of volatile acids can easily overwhelm methanogens leading to accumulation of volatile acids, eventually resulting in a drop in digester pH. These impacts are exacerbated in systems with high FOG loading.

However, FOG can be made into an excellent food substrate for anaerobic digestion, due to their high COD content, as well as their high potential to generate large quantities of acetate or acetic acid. It just needs a little processing to get there.

Ana-Zyme G and Anaerobic Grease

Ana-Zyme G acts as a catalyst to naturally occurring bacteria to aid in the breakdown of FOG, which prevents the overloading of methanogens and frees up this new food source. Ana-Zyme G does this by targeting triglycerides and long-chain fatty acids to convert them evenly into short-chain fatty acids. This stabilization of fatty acid degradation allows methanogens to better acclimate to fatty acid loading. They are then able quickly uptake these fatty acids, reducing the chances of volatile fatty acid accumulation. In addition, Ana-Zyme G prevents the accumulation of undegraded fats in a system, which enhances volatile solids destruction, particularly in systems with limited mixing such as lagoons.


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