Cold Weather Effects on Nitrification & Ammonia Removal

by Deborah Lee, Saylor Gilbert, and Tyler Benz

In this event, Lead Microbiologist and Nitrifier Expert, Deborah Lee, Research Scientist, Saylor Gilbert, and Technical Consultant, Tyler Benz, will answer audience questions relating to nitrification and ammonia removal and discuss considerations for the best nitrification. For additional information or questions, please contact us at 888-757-9577 or


00:18 Overview
00:58 Nitrification in the Activated Sludge Process
01:23 Organic Nitrogen
01:47 Bacteria in Wastewater Systems
03:32 Nitrifying Bacteria
04:26 Nitrifiers and Floc
05:06 Nitrite Lock
06:16 Optimal Nitrification Conditions
07:21 Temperature
08:23 Cold Temp Shock 20° C to 7° C
12:16 Combining PAA and Quat Toxicity
14:56 MLSS COD Reduction and Ammonia Reduction
16:36 VitaStim Nitrifiers after Quat Toxicity
18:49 Dynamic Duo
19:16 CounterQuat
20:10 Microanalysis & Filament Origins
20:45 Q&A

wastewater testing sample kit

Dynamic Duo

  • Restores populations of nitrifying and nitrite reducing organisms
  • Works great in water temperature as low as 39°F
  • For use in aerobic wastewater systems
  • Ships next day and guarantees full recovery in 6-10 days


  • Mitigate potential upsets and protect bacteria from toxins
  • Works against Quats, PAAs, and other cleaning agents
  • Great for systems with frequent cleanouts
wastewater testing sample kit

Microanalysis & Filament Origins

  • Identification of major and minor filaments
  • Precise explanation of filament presence and origin
  • Overview of floc structure, sludge age, and oxygen penetration
  • Treatment and process recommendations